Labeling of nucleic acids with fluorescent markers

Details Labeling of nucleic acids with fluorescent markers Labeling of nucleic acids with fluorescent markers

C - Chemistry, Metallurgy

12

N

195/1.12, 150/8.

C12N 15/00 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2007431

ABSTRACT OF THE INVENTION The present invention relates to DNA markers and, particularly, nucleic acid labeling techniques. More specifically, this invention contemplates a protocol which permits the covalent introduction of single or multiple fluorescent markers or other probes into DNA fragments and oligodeoxynucleotides. The instant technique, particularly employing multiple fluorescent markers, allows high sensitivity detection of nucleic acids (without the use of sophisticated detection devices) in the low femtomolar (10-15 mol) range and additionally permits the placement of markers and probes at specific locations within the macromolecule. The present invention can be used with high detection sensitivity for DNA sequencing and hybridization procedures including a host of diagnostic and therapeutic procedures. The present technique can be employed as a tool for the study of nucleic acid dynamics through recognition and evaluation of fluorescence energy transfer and electron spin resonance, and the study of structure, conformation and dynamics of biopolymers. Specific labeling procedures allow the introduction of a probe or other entity for the location of desired sequences or the delivery of the probe to a specific sequence. This process is fundamental to the emerging fields of DNA diagnostics and therapeutics.

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