Labeling of rapamycin using rapamycin-specific methylases

C - Chemistry – Metallurgy – 12 – P

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C12P 17/18 (2006.01) C07D 498/18 (2006.01)

Patent

CA 2562164

A method for rapamycin-specific labeling using rapI, rapM and/or rapQ enzymes is described. Also are methods for generating crude enzyme extracts useful in the method of the invention. Uses of the specifically labeled rapamycin as diagnostic tools are provided.

L'invention concerne un procédé de marquage spécifique de la rapamycine mettant en oeuvre les enzymes rapI, rapM et/ou rapQ. L'invention concerne également des procédés de production d'extraits enzymatiques bruts utilisés dans ledit procédé. L'invention concerne encore des utilisations de la rapamycine spécifiquement marquée en tant qu'instrument diagnostique.

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