Labelling and sequencing of nucleic acids

Details Labelling and sequencing of nucleic acids Labelling and sequencing of nucleic acids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2583277

The invention provides for methods of end labelling a ds DNA molecule with a bar-code as well as adaptor mediated methodology for creating single stranded overhanging ends, lengthening single stranded overhanging ends, controlled size reduction of labelled ds DNA molecules, and adaptor mediated sequencing. Also disclosed are methods for parallel sequencing of multiple ds DNA molecules in a mixture by visual means.

L'invention concerne des procédés d'étiquetage final d'une molécule ADN ds avec un code barre ainsi qu'une méthodologie à médiation par un adaptateur pour créer des extrémités en saillie monocatenaires, pour allonger les extrémités en saillie monocatenaires, pour procéder à la réduction contrôlée de la taille de molécules ADN ds étiquetées et au séquençage à médiation par un adaptateur. L'invention concerne des procédés de séquençage parallèle de molécules ADN ds multiples dans un mélange par un moyen visuel.

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