Labelling composition and method

Details Labelling composition and method Labelling composition and method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2300865

A labelling composition comprises a random mixture of oligonucleotides which are 6-mers to 8-mers, said composition present in a dry state. A method of making labelled probes for a nucleic acid template comprises incubating the template under chain extension conditions with the labelling composition. The use of 6-mers to 8-mers reduces self-annealing, which is a problem with 9-mers in a dried state.

L'invention concerne une composition de marquage comprenant un mélange aléatoire d'oligonucléotides qui sont des 6-, 7- et 8-mères, cette composition étant présente à l'état sec. L'invention concerne également une technique de préparation de sondes marquées pour une matrice d'acide nucléique, consistant à incuber la matrice avec la composition de marquage dans des conditions propres à entraîner un allongement de la chaîne. L'utilisation de 6-, 7- et 8-mères permet de réduire l'auto-hybridation qui représente un problème avec les 9-mères à l'état sec.

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