Labelling of oligonucleotides

Details Labelling of oligonucleotides Labelling of oligonucleotides

C - Chemistry, Metallurgy

07

H

195/1.26, 150/8.

C07H 21/00 (2006.01) C07H 19/06 (2006.01) C07H 19/073 (2006.01) C07H 19/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1278755

ABSTRACT OF THE DISCLOSURE A method is provided of making an oligo- nucleotide which comprises hybridizing (a) a shorter fragment of the desired oligonucleotide with (b) a nucleic acid fragment longer than (a) and complementary to the desired oligonucleotide, one of (a) and (b) being limited to an organic radical which differentiates its physical properties relative to the other of (a) and (b), contacting the hybridized material with an enzyme and nucleoside triphosphates whereby the shorter fragment is extended in one direction until it is coterminal with the complementary nucleic acid fragment, denaturing the hybridized material and separating lengthened (A) from (b).

500968

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