Laboratory examination of a body fluid or tissue sample

Details Laboratory examination of a body fluid or tissue sample Laboratory examination of a body fluid or tissue sample

G - Physics

01

N

G01N 33/50 (2006.01) C12P 13/24 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2370427

The invention relates to a laboratory examination of a body fluid or tissue sample in which a quantitatively analytical detection of cis-hydroxyproline and the derivatives thereof is carried out. An analysis kit is provided which, using the HPLC method, is suited for determining the quantity of cis- hydroxyproline and derivatives thereof in the sample. Said analysis kit comprises HCl (10 M), NaOH (16 M), Na2CO3 (4 M), ortho-phthaldialdehyde, an aqueous phosphate buffer and dabsyl chloride.

L'invention concerne un procédé d'examen en laboratoire d'un échantillon de liquide organique ou de tissu, selon lequel il est prévu une mise en évidence analytique quantitative de la présence de cis-hydroxyproline et de ses dérivés. Un coffret d'analyse approprié pour déterminer la teneur de l'échantillon en cis-hydroxyproline et celle de ses dérivés comprend HCl (10 M), NaOH (16 M), Na¿2?CO¿3? (4 M), ortho-phtaldialdéhyde, tampon de phosphate aqueux et chlorure de dabsyle.

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