Lac expression system

Details Lac expression system Lac expression system

C - Chemistry, Metallurgy

12

N

C12N 15/72 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01)

Patent

CA 2706918

Provided herein is a nucleic acid comprising a mutant lac operator operably linked to a gene of interest, a host cell comprising the nucleic acid, and a method of using the host cell to express the gene of interest. Also provided is a recA-mediated cloning method.

L'invention porte sur un acide nucléique comprenant un opérateur lac mutant lié de façon fonctionnelle à un gène d'intérêt, sur une cellule hôte comprenant l'acide nucléique, et sur un procédé d'utilisation de la cellule hôte pour exprimer le gène d'intérêt. L'invention porte également sur un procédé de clonage à médiation par recA.

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