C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A21D 8/04 (2006.01) A23C 9/12 (2006.01) A23C 9/152 (2006.01) A23C 17/00 (2006.01) A23K 1/165 (2006.01) A23L 1/03 (2006.01) A23L 1/30 (2006.01) A23L 2/84 (2006.01) A23L 3/3463 (2006.01) A24B 15/20 (2006.01) A24B 15/24 (2006.01) A61P 31/00 (2006.01) B27K 5/00 (2006.01) C07K 16/40 (2006.01) C07K 17/00 (2006.01) C07K 19/00 (2006.01) C11D 3/386 (2006.01) C12C 5/02 (2006.01) C12C 7/00 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01)
Patent
CA 2535526
The invention provides laccases, polynucleotides encoding these enzymes, the use of such polynucleotides and polypeptides. In one aspect, the invention relates to the enzymatic production of nootkatone by way of the conversion of valencene using proteins having a laccase activity, e.g., a novel laccase of the invention. In one aspect, the invention provides methods of depolymerizing lignin, e.g., in a pulp or paper manufacturing process, using a polypeptide of the invention. In another aspect, the invention provides methods for oxidizing products that can be mediators of laccase-catalyzed oxidation reactions, e.g., 2,2-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS), 1- hydroxybenzotriazole (HBT), 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO), dimethoxyphenol, and the like.
La présente invention concerne des laccases, des polynucléotides codant pour ces enzymes, ainsi que l'utilisation de tels polynucléotides et de polypeptides. Un aspect de cette invention concerne la production enzymatique de nootkatone par conversion du valencène au moyen de protéines présentant une activité de laccase, par exemple, une nouvelle laccase décrite dans cette invention. Un autre aspect de cette invention concerne des procédés permettant de dépolymériser la lignine, par exemple, lors d'un processus de fabrication de papier ou de pulpe, au moyen d'un polypeptide décrit dans cette invention. Un autre aspect de cette invention concerne des procédés permettant d'oxyder des produits qui peuvent être des médiateurs de réactions d'oxydation catalysées par laccases, par exemple, 2,2-azinobis-(3-éthylbenzthiazoline-6-sulfonate) (ABTS), 1- hydroxybenzotriazole (HBT), 2,2,6,6-tetraméthylpipéridin-1-yloxy (TEMPO), diméthoxyphénol, etc.
Gray Kevin A.
Hiraiwa Masao
Hitchman Tim
Phillips Yoko
Robertson Dan E.
Diversa Corporation
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
Verenium Corporation
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