Lactic acid bacterial starter cultures and compositions thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) A23C 9/123 (2006.01) C12N 1/20 (2006.01) C12N 9/02 (2006.01) C12P 7/56 (2006.01) C12P 39/00 (2006.01)

Patent

CA 2291878

Methods of enhancing the growth rate and/or controlling the metabolic activity of lactic acid bacteria and of improving the shelf life and/or the quality of an edible product using lactic acid bacterial organisms which are defective in their pyruvate metabolism. There is also provided starter culture compositions comprising such defective lactic acid bacteria as helper organisms and lactic acid bacterial starter culture strains. Useful helper organisms are Lactococcus strains which are defective with respect to pyruvate formate lyase (Pfl) and/or lactate dehydrogenase (Ldh) activity. The helper organisms may overexpress a gene coding for an NAD+ regenerating enzyme such as NADH oxidase encoded by nox gene.

Cette invention se rapporte à des procédés pour augmenter la vitesse de croissance et/ou contrôler l'activité métabolique de bactéries d'acide lactique et pour améliorer la durée de conservation et/ou la qualité d'un produit comestible, au moyen d'organismes bactériens d'acide lactique qui sont déficients dans leur métabolism du pyruvate. Cette invention présente également des compositions de cultures starter comprenant ces bactéries d'acide lactique déficientes comme organismes auxiliaires et des souches de cultures starter bactériennes d'acide lactique. Les organismes auxiliaires utiles sont les souches de Lactococcus qui sont déficientes par rapport à l'activité de la pyruvate formate lyase (Pfl) et/ou de la lactate déshydrogénase (Ldh). Ces organimses auxiliaires peuvent surexprimer un gène codant pour une enzyme de régénération de NAD?+¿, tel que la NADH oxydase codée par le gène nox.

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