Lactobacillus helveticus bacterium having high capability of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) A23C 9/123 (2006.01) A23C 9/137 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2304985

There is provided a fermented milk product that contains lactic acid bacteria capable of producing a large amount of lactotripeptide and a large amount of active ingredient having hypotensive activity and anti-stress effect, and that can be taken pleasantly as foods or beverages. Lactic acid bacteria of Lactobacillus helveticus having specific bacteriological properties, the bacteria, when cultured in a medium of animal milk containing 9wt% solid of non-fat milk, producing tripeptides Val-Pro-Pro and Ile-Pro-Pro in an amount of 60µg in terms of Val-Pro-Pro per ml medium, and the bacteria exhibiting extracellular proteinase activity of not lower than 400U/OD590. Lactobacillus helveticus CM4 strain (deposited at National Institute of Bioscience and Human-Technology Agency of Industrial Science and Technology, deposition number FERM BP-6060). A fermented milk product obtained by fermenting an animal milk with these lactic acid bacteria.

L'invention concerne une souche de bactérie lactique capable de produire une grande quantité de lactotripeptides; un produit laitier fermenté qui contient plusieurs composants ayant des activités hypotensives et apaisantes et qui se mange et s'avale facilement. L'invention porte plus spécifiquement sur une bactérie lactique du genre Lactobacillus helveticus, c.a.d. la souche CM4 de Lactobacillus helveticus (déposée sous le numéro d'entrée FERM BP-6060 par le National Institute of Bioscience et Human Technology). Ladite souche se caractérise en ce qu'elle a des propriétés mycologiques particulières, qu'elle produit les tripeptides Val-Pro-Pro et/ou Ile-Pro-Pro à raison d'au moins 60 mu g/ml en termes de Val-Pro-Pro lorsqu'elle est mise en culture dans un milieu constitué de lait animal, qu'elle contient au moins 9 % en poids (en termes de teneur en extrait sec) de lait non gras, et qu'elle présente une activité de protéase extracellulaire d'au moins 400 U/OD590. L'invention se rapporte encore à un lait fermenté que l'on prépare en faisant fermenter du lait animal avec ladite bactérie lactique.

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