Ladder assembly and system for generating diversity

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/09 (2006.01)

Patent

CA 2590245

The present invention provides novel methods of generating a nucleic acid molecule. In certain embodiments, a double stranded nucleic acid chunk is generated from a ladder complex comprising partially complementary oligonucleotides, which chunk is combined with a nucleic acid acceptor molecule. In certain embodiments, the assembled chunk/nucleic acid acceptor molecule complex may be propagated in vivo or in vitro. The present invention also provides improved systems for generating a plurality of nucleic acid molecules that differ at one or more nucleotide positions. In certain embodiments, the plurality of nucleic acid molecules encodes a polypeptide or portion of a polypeptide.

La présente invention concerne de nouveaux procédés de génération d~une molécule d~acide nucléique. Dans certains modes de réalisation, un fragment d~acide nucléique double brin est généré à partir d~un complexe en échelle comprenant des oligonucléotides partiellement complémentaires, ledit fragment étant combiné à une molécule d~accepteur d~acide nucléique. Dans certains modes de réalisation, le complexe formé du fragment d~acide nucléique et de la molécule d~accepteur d~acide nucléique peut être propagé in vivo ouin vitro. La présente invention concerne également des procédés améliorés permettant de générer une pluralité de molécules d~acide nucléique différant les unes des autres d~au moins une position de nucléotide. Dans certains modes de réalisation, la pluralité de molécules d~acide nucléique code un polypeptide ou une partie d~un polypeptide.

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