Lambda integrase mediated recombination in plants

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2480642

The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including for example, dicotyledonous and monocotyledonous plant cells. The present disclosure provides a method for effecting site-specific recombination of DNA within a plant cell, comprising : introducing into the plant cell a target nucleotide sequence comprising a first Int recognition site ; introducing into the plant cell a donor nucleotide sequence comprising a second Int recognition site ; and introducing into the plant cell an integrase or integrase complex.

La présente invention concerne des méthodes permettant d'obtenir l'intégration ciblée d'une molécule d'ADN dans le génome d'une cellule hôte au moyen d'une recombinase. On peut utiliser ces méthodes avec diverses cellules hôtes, y compris par exemple, des cellules végétales dicotylédones et monocotylédones. Cette invention a également trait à une méthode permettant de réaliser une recombinaison d'ADN spécifique de sites au sein d'une cellule végétale, ladite méthode consistant à introduire dans la cellule végétale une séquence de nucléotides cible contenant un premier site de reconnaissance Int, puis, une séquence de nucléotides donatrice renfermant un second site de reconnaissance Int, et, enfin, une intégrase ou un complexe d'intégrases.

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