Lambda integrase mutein for use in recombination

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/22 (2006.01)

Patent

CA 2522166

Provided is a lambda integrase mutein for use in recombination. This mutein finds utility, for example in application to artificial chromosomes, including ACes, that have been engineered to contain available sites for site- specific, recombination-directed integration of DNA of interest as provided herein. These artificial chromosomes permit tractable, efficient, rational engineering of the chromosome.

L'invention porte sur une mutéine d'intégrase lambda utilisée en recombinaison. L'une des applications utiles de cette mutéine est le chromosome artificiel, y compris Aces. Ces chromosomes ont été conçus pour subir une recombinaison-intégration d'un ADN d'intérêt décrit dans l'invention, ayant lieu à un site spécifique. Ces chromosomes artificiels permettent un génie adaptable, efficient et rationnel du chromosome.

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