Large scale disposable bioreactor

Details Large scale disposable bioreactor Large scale disposable bioreactor

C - Chemistry, Metallurgy

12

M

C12M 1/00 (2006.01) C12M 1/04 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2685701

A reusable, disposable device for culturing plant tissues or cells including a non-rigid container having dimensions and gas exchange ports designed for maintaining oxygen saturation and shear forces suitable for culturing plant tissue or cells in 400 liters or more of culture medium is provided. Also provided are methods for producing a catalytically active human recombinant protein in a plant cell, using the disposable device of one of the embodiments of the instant specification.

L'invention concerne un dispositif jetable, réutilisable pour mettre en culture des tissus végétaux ou cellules végétales, le dispositif comprenant un conteneur non rigide ayant des dimensions et des orifices d'échange de gaz mis au point pour maintenir une saturation de l'oxygène et des forces de cisaillement appropriées pour mettre en culture un tissu végétal ou des cellules végétales dans 400 litres ou plus de milieu de culture. L'invention concerne également des procédés pour produire une protéine recombinante humaine catalytiquement active dans une cellule végétale, à l'aide du dispositif jetable d'un des modes de réalisation de la présente invention.

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