Large-scale electroporation plates, systems, and methods of use

Details Large-scale electroporation plates, systems, and methods of use Large-scale electroporation plates, systems, and methods of use

C - Chemistry, Metallurgy

12

N

C12N 13/00 (2006.01) C12M 1/18 (2006.01) C12M 1/42 (2006.01) C12M 3/00 (2006.01) C12N 1/00 (2006.01) C12N 1/34 (2006.01)

Patent

CA 2508358

Described are electroporation plates (2) that comprise a plurality of energizable electroporation wells (4) or chambers arrayed in a solid substrate, wherein at least two of the wells (4) in the plate (2) can be independently energized. Each well (4) contains at least two electroporation electrodes (12, 14) disposed therein, and serves as the vessel in which an individual electroporation reaction can be conducted. Also, described are electroporation systems that use electroporation plates (2) according to the invention, as well as methods of using such electroporation plates (2) and systems, for example, to optimize electroporation conditions.

L'invention concerne des plaques (2) d'électroporation qui comprennent une pluralité de puits (4) ou chambres d'électroporation pouvant être excités, mis en réseau dans un substrat solide. Au moins deux des puits (4) dans la plaque peuvent être excités indépendamment. Chaque puits (4) contient au moins deux électrodes (12, 14) d'électroporation disposées à l'intérieur de ces derniers, et le puits est utilisé en tant que récipient, dans lequel une réaction individuelle d'électroporation peut être effectuée. L'invention concerne également des systèmes d'électroporation qui utilisent des systèmes d'électroporation qui eux-mêmes utilisent des plaques (2) d'électroporation selon l'invention, ainsi que des procédés d'utilisation desdites plaques (2) d'électroporation et des systèmes, par exemple, utilisés pour optimiser les conditions d'électroporation.

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