Large scale microbial culture method

Details Large scale microbial culture method Large scale microbial culture method

C - Chemistry, Metallurgy

12

P

C12P 7/46 (2006.01)

Patent

CA 2709515

The invention generally relates to a new culture method for producing high levels of a metabolite, such as succinic acid. The culture method uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having < 5% partial pressure of oxygen, and the production of high levels of succinate under oxygen deprived conditions. The method can be performed in a single reactor, and is amenable to efficient scale up.

L'invention porte d'une manière générale sur un nouveau procédé de culture pour produire des taux élevés d'un métabolite, tel que l'acide succinique. Ce procédé de culture comprend une culture riche en oxygène sans ajustement de pH pour augmenter la biomasse, une acclimatation dans des conditions pauvres en oxygène comprenant une pression partielle < 5 % d'oxygène, et la production de taux élevés de succinate dans des conditions privées d'oxygène. Ce procédé peut être effectué dans un seul réacteur et on peut le faire passer à une plus grande échelle de manière efficace.

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