Large scale production of dna probes

Details Large scale production of dna probes Large scale production of dna probes

C - Chemistry, Metallurgy

07

H

195/1.12

C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1264452

ABSTRACT A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making ana].ytical probes.

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