Large-scale production of soluble hyaluronidase

Details Large-scale production of soluble hyaluronidase Large-scale production of soluble hyaluronidase

C - Chemistry, Metallurgy

12

N

C12N 9/26 (2006.01)

Patent

CA 2717383

Provided are methods for preparing large-scale preparations of soluble hyaluronidases. The methods employ cells that contain a plurality of active copies of nucleic acid encoding the soluble hyaluronidase and a plurality of feedings and temperature changes, whereby the encoded soluble hyaluronidase is secreted into the cell culture medium.

L'invention porte sur des procédés d'obtention de préparations à grande échelle d'hyaluronidases solubles. Les procédés emploient des cellules qui contiennent une pluralité de copies actives d'acide nucléique codant pour la hyaluronidase soluble et une pluralité d'alimentations et de changements de température, ce par quoi l'hyaluronidase soluble codée est sécrétée dans le milieu de culture cellulaire.

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