Latent human tuberculosis model, diagnostic antigens, and...

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G01N 33/569 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2433069

Provided herein is an <i>in vitro</i> granuloma model and methods of its use. Methods of detecting and/or diagnosing latent tuberculosis in a subject are also provided, as are latency-specific antigens (and antibodies thereto), such as .alpha.-crystallin, and methods of identifying and using such molecules. Also provided are immunostimulatory compositions, for instance for use in eliciting an immune response in a subject, such as an immune response to a latent tuberculosis infection. Kits for carrying out the provided methods are also described.

L'invention concerne un modèle de granuloma in vitro et ses méthodes d'utilisation. L'invention concerne également des méthodes de détection et/ou de diagnostic de la tuberculose latente chez un sujet, des antigènes spécifiques de latence (et les anticorps de ceux-ci), par exemple de type .alpha.-cristallin, et des méthodes d'identification et d'utilisation de ces molécules. Par ailleurs, l'invention concerne des composition immunostimulantes, utilisées notamment pour provoquer une réponse immunitaire chez un sujet, par exemple une réponse immunitaire vis à vis d'une infection tuberculeuse latente. Enfin, l'invention concerne des kits d'application de ces méthodes.

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