Lateral flow, non-bibulous membrane protocols

G - Physics – 01 – N

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G01N 33/558 (2006.01) G01N 33/543 (2006.01) G01N 37/00 (2006.01)

Patent

CA 1313616

-60- Abstract A method and apparatus for conducting specific binding pair assays, such as immunoassays, is described. A porous membrane capable of non-bibulous lateral flow is used as assay substrate; a member of the binding pair is affixed in an indicator zone defined in the substrate. The sample is applied at a position distant from the indi- cator zone and permitted to flow laterally through the zone; any analyte in the sample is complexed by the af- fixed specific binding member, and detected. A novel method of detection employs entrapment of observable particle in the complex. Blood is a particularly pre- ferred sample as the red blood cells can be used as the observable particles for detection of the complex.

568070

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