Lawsonia intracellularis subunit vaccine

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/02 (2006.01) C07K 14/195 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2536989

The present invention relates i.a. to nucleic acid sequences encoding novel Lawsonia intracellularis proteins. It furthermore relates to DNA fragments, recombinant DNA molecules and live recombinant carriers comprising these sequences. Also it relates to host cells comprising such nucleic acid sequences, DNA fragments, recombinant DNA molecules and live recombinant carriers. Moreover, the invention relates to proteins encoded by these nucleotide sequences and to their use for the manufacturing of vaccines. The invention also relates to vaccines for combating Lawsonia intracellularis infections and methods for the preparation thereof. Finally the invention relates to diagnostic tests for the detection of Lawsonia intracellularis DNA, the detection of Lawsonia intracellularis antigens and of antibodies against Lawsonia intracellularis.

L'invention se rapporte, entre autres, à des séquences d'acide nucléique qui codent de nouvelles protéines Lawsonia intracellularis. Cette invention concerne également des fragments d'ADN, des molécules d'ADN recombinées et des porteurs recombinés vivants comprenant lesdites séquences. La présente invention concerne aussi des cellules hôtes comprenant lesdites séquences d'acide nucléique, des fragments d'ADN, des molécules d'ADN recombinées et des porteurs recombinés vivants. Cette invention se rapporte par ailleurs à des protéines codées par lesdites séquences nucléotidiques, ainsi qu'à leur utilisation pour produire des vaccins. Cette invention concerne en outre des vaccins servant à lutter contre des infections par Lawsonia intracellularis, ainsi que des procédés de préparation de ces vaccins. Enfin, la présente invention concerne des épreuves diagnostiques servant à détecter de l'ADN de Lawsonia intracellularis, des antigènes de Lawsonia intracellularis, et des anticorps dirigés contre Lawsonia intracellularis.

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