Layered immunoassay using antibodies bound to transport...

Details Layered immunoassay using antibodies bound to transport... Layered immunoassay using antibodies bound to transport...

G - Physics

01

N

167/45

G01N 33/531 (2006.01) C12M 1/107 (2006.01) G01N 33/537 (2006.01) G01N 33/558 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1293442

LAYERED IMMUNOASSAY USING ANTIBODIES BOUND TO TRANSPORT PARTICLES TO DETERMINE THE PRESENCE OF AN ANTIGEN ABSTRACT OF THE INVENTION A device is provided for determining the presence of an antigen which comprises a trapping zone, which contains material capable of capturing free flowing enzyme linked antibodies, but not antibodies bound to a transport particle which flows freely through the trapping zone into the substrate zone, and a substrate zone which contains material capable of reacting with enzyme-linked antibodies to produce a reaction which indicates the presence of antibodies. A method of determining the presence of an antigen is provided wherein a sample is mixed with two classes of antibodies which are specific for the antigen being tested for, but which react with different antigen domains, wherein the mixture consists of class one antibodies bound to a transport particle which flows freely through the trapping zone and class two enzyme-linked antibodies which are incapable, unless bound to the transport particles, of flowing freely through the trapping zone. In the presence of the antigen being tested for, both classes of antibodies bind to the antigen and flow through the trapping zone into the substrate zone, wherein a reaction takes place to indicate the presence of the antigen.

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