Leader sequence for the production of recombinant proteins

Details Leader sequence for the production of recombinant proteins Leader sequence for the production of recombinant proteins

C - Chemistry, Metallurgy

12

N

530/15.12, 195/1

C12N 15/62 (2006.01) C07K 14/485 (2006.01) C12N 15/67 (2006.01)

Patent

CA 1334943

Disclosed is a novel polypeptide useful as a leader or trailer peptide moiety in recombinant DNA protein production techniques involving fused protein methodology. The polypeptide comprises an amphiphilic helix designed at the DNA level to have hydrophilic charged amino acid residues on one side of the barrel of the helix and nonpolar amino acid residues on the other side of the barrel of the helix. When DNA encoding the helix is attached to a gene encoding a protein of interest, high level expression is achieved and inclusion bodies are spontaneously formed. The inclusion bodies may be collected and purified easily by altering the ionic strength and/or pH of media used to dissolve the inclusion bodies. After purification, the fused protein is cleaved to separate the amphiphilic helix from the product.

561914

Affiliated with

Also associated with

Rating

Leader sequence for the production of recombinant proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Leader sequence for the production of recombinant proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Leader sequence for the production of recombinant proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1195626