Leader sequences for use in production of proteins

Details Leader sequences for use in production of proteins Leader sequences for use in production of proteins

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/715 (2006.01) C12N 5/10 (2006.01) C12N 15/58 (2006.01)

Patent

CA 2537340

This invention encompasses novel leader sequences for production of proteins. More specifically, the invention relates to DNA constructs encoding leader sequences comprising an immunoglobulin signal peptide fused to a tissue -type plasminogen activator propeptide, and to DNA constructs encoding leader sequences comprising a truncated human tissue -type plasminogen activator propeptide. The invention further relates to the use of these DNA constructs for producing proteins in mammalian cells.

Cette invention concerne de nouvelles séquences de tête utilisées dans la production de protéines. De manière plus spécifique, cette invention concerne des constructions d'ADN codant des séquences de tête comprenant un peptide signal d'immunoglobuline fusionné à un propeptide de l'activateur tissulaire du plasminogène ainsi que des constructions d'ADN codant des séquences de tête comprenant un propeptide de l'activateur tissulaire du plasminogène humain tronqué. Cette invention concerne en outre l'utilisation de ces constructions d'ADN pour produire des protéines dans des cellules mammifères.

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