Lectins for analyzing sugar chains and method of using the same

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/04 (2006.01) C07K 14/42 (2006.01) C12N 15/09 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2438952

It is expected that lectins of a needed number of types appropriately corresponding the variation of sugar chains, if available, are highly useful as tools for analyzing various sugar chains. From a lectin library containing plural types of lectins or an arbitrary mass of lectins containing plural types of lectins, lectins needed for analyzing sugar chains are selected to thereby construct a lectin sublibrary. Using the lectin sublibrary thus constructed, cells having sugar chains are identified. Moreover, an indication method whereby cells can be conveniently identified is provided.

Selon l'invention, les lectines de plusieurs types correspondant aux changements survenant dans les chaînes glucidiques, si elles sont disponibles, sont très utiles comme instruments d'analyse des diverses chaînes glucidiques. On choisit les lectines nécessaires à l'analyse des chaînes glucidiques à partir d'une banque de lectines contenant plusieurs types de lectines ou un volume arbitraire de lectines contenant plusieurs types de lectines, ce qui permet d'établir une sous-banque de lectines. On identifie les cellules comportant des chaînes glucidiques au moyen de la sous-banque ainsi établie. L'invention concerne également un procédé d'identification aisée de cellules.

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