Lentiviral vector system for high quantity screening

Details Lentiviral vector system for high quantity screening Lentiviral vector system for high quantity screening

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01) A61K 48/00 (2006.01) C07K 14/145 (2006.01) C07K 14/155 (2006.01) C07K 14/52 (2006.01) C07K 16/00 (2006.01) C12N 7/04 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2366054

A method of screening for a target molecule from a group of potential target molecules is described. This method involves using a library of lentiviral vectors where the members encode the group of target molecules, then transducing a group of cells and screening the transduced cell for a desired phenotype. The cell(s) displaying the desired phenotype is selected and the target molecule is identified.

L'invention concerne une méthode de criblage d'une une molécule cible appartenant à un groupe de molécules cibles potentielles. Ladite méthode consiste, d'abord, à utiliser une bibliothèque de vecteur lentiviraux, les membres codant le groupe de molécules cibles, puis à transduire un groupe de cellules et enfin à sélectionner la cellule transduite pour un phénotype déterminé. La/les cellules contenant le phénotype recherché est/sont sélectionnée(s) puis la molécule est identifiée.

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