Lettuce infectious yellows virus genes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/40 (2006.01) A01H 5/00 (2006.01) C07K 14/08 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12N 15/84 (2006.01)

Patent

CA 2166786

The present invention provides an isolated nucleic acid which contains a nucleotide sequence which encodes at least one of five LIYV proteins: the coat protein, the heat shock protein-70, RNA polymerase, the protein encoded by the gene positioned at ORF 3 of LIYV RNA1, and the protein encoded by the gene positioned at ORF 6 of LYIV RNA2 where at least a portion of the nucleotide sequence for at least one of these proteins, either in the sense or the antisense orientation, is operably linked to genetic regulatory sequences necessary for gene expression to form plant transformation vectors. These vectors are then used to prepare transgenic seeds and plants.

L'invention porte sur un acide nucléique isolé comportant une séquence nucléotidique codant pour au moins l'une des cinq protéines du LIYV (virus infectieux jaune de la laitue) suivantes; la protéine de coque, la protéine choc thermique 70, une ARN-polymérase, la protéine codée par le gène positionné en ORF 3 de l'ARN 1 du LIYV, et la protéine codée par le gène positionné en ORF 6 de l'ARN 2 du LIYV. Une partie au moins de la séquence nucléotidique est liée opérationnellement à des séquences génétiques régulatrices nécessaires à l'expression de gènes en vue de la formation de vecteurs de transformation de plantes, lesquels vecteurs servent alors à la préparation de graines et de plantes transgéniques.

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