Leucine aminopeptidases produced recombinantly from...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/57 (2006.01) C12N 1/15 (2006.01) C12N 9/48 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2226545

The invention relates to a recombinant deoxyribonucleic acid (DNA) which can be isolated from Aspergillus soyae, which is coded for a leucine aminopeptidase (LAP) and has a nucleotide sequence corresponding to that given in SEQ ID NO:1 for ripe LAP or one derived thereform, which hybridises in stringent conditions with the nucleotide sequence given in SEQ ID NO:1 for ripe LAP. The invetion also relates to vectors, transformed host organisms and a process for the production of LAP. Enzyme products for the production of protein hydrolysates and correspondingly produced protein hydrolysates also form part of the invention.

L'invention concerne un acide désoxyribonucléique (ADN) de recombinaison, que l'on peut isoler à partir de l'aspergillus sojae, qui est codé pour une leucin-aminopeptidase (LAP) et a une séquence nucléotide correspondant à la séquence nucléotide indiquée dans SEQ ID NO:1 pour la LAP arrivée à maturation ou bien une séquence nucléotide dérivée, qui s'hybride dans des conditions stringentes avec la séquence nucléotide indiquée dans SEQ ID NO:1 pour la LAP arrivée à maturation. L'invention concerne également des vecteurs, des organismes hôtes transformés ainsi qu'un procédé de production de LAP. Des produits enzymatiques pour la production d'hydrolysats protéiques ainsi que les hydrolysats protéiques en question font également partie intégrante de l'invention.

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