Leukocyte differentiation composition and method

Details Leukocyte differentiation composition and method Leukocyte differentiation composition and method

G - Physics

01

N

150/13, 150/3

G01N 33/50 (2006.01)

Patent

CA 1255197

ABSTRACT A composition and method are disclosed for differentiat- ing white blood cells. The composition comprises at least one surfactant and at least one dilute acid which together selectively strip the cytoplasm from certain classes of white blood cells and not others. More particularly, the composi- tion causes lysis of lymphocytes, monocytes, eosinophils and neutrophils, but not basophils. Thus, basophils are differ- entiated from other PMN sub-classes by their appearance as intact cells. The composition and method totally avoid the need for dye preparation and the vagaries of staining tech- niques and can be used either manually or with instrumenta- tion.

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