Leukocytes and hemoglobin in blood

G - Physics – 01 – N

Patent

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150/3

G01N 33/72 (2006.01) G01N 33/50 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2027450

A reagent for measuring the hemoglobin concentration and counting the number of leukocytes in a blood sample, which contains at least one of a quaternary ammonium salt or a pyridinium salt, having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglo- bin and at least one of cationic, nonionic or amphoteric surfactants or an oxidant capable of oxidizing heme in hemoglobin, said reagent being capable of dividing leuko- cytes into two or three fractions, for example, a fraction of lymphocytes, a fraction of monocytes, eosinophils and basophils, and a fraction of neutrophils.

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