C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/02 (2006.01) C12P 7/26 (2006.01) C12P 21/02 (2006.01) C12P 41/00 (2006.01)
Patent
CA 2297440
A levodione reductase having the following physico-chemical properties is provided: molecular weight: 142,000 - 155,000 ~ 10.000 (consisting of four homologous subunits having a molecular weight of 36,000 ~ 5,000); co-factor: nicotinamide adenine dinucleotide (NAD/NADH); substrate specificity: active on levodione; optimum temperature: 15 - 20°C at pH 7.0; optimum pH: 7.5; and activator: K+, Cs+, Rb+, Na+ and NH4+. Said levodione reductase, which is capable of producing actinol, an important intermediate for the production of zeaxanthin, from levodione, can be produced from a microorganism belonging to the genus Corynebacterium, preferably the microorganism Corynebacterium aquaticum AKU 611 (FERM BP-6448) or a functional equivalent, subculture, mutant or variant thereof. Further inventive aspects are a process for producing the levodione reductase comprising cultivating a microorganism of the genus Corynebacterium in an aqueous medium under aerobic conditions, disrupting the cells of the microorganism and isolating and purifiying the levodione reductase from the cell-free extract of the disrupted cells of the microorganism, and a process for producing actinol from levodione comprising contacting levodione with the levodione reductase in the presence of the reduced form of nicotinamide adenine dinucleotide or a cell-free extract of said microorganism, and in each case isolating the resulting actinol from the reaction mixture.
Nakamori Shigeru
Shimizu Sakayu
Wada Masaru
Dsm Ip Assets B.v.
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
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