Li-key hybrid peptides that modulate the immune response to...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/11 (2006.01) A61K 39/145 (2006.01) A61P 37/04 (2006.01) C07K 14/47 (2006.01) C07K 19/00 (2006.01) C12Q 1/02 (2006.01) C40B 30/04 (2006.01) C40B 30/06 (2006.01)

Patent

CA 2750922

The present invention provides an MHC class II antigen presentation enhancing hybrid polypeptide. The hybrid has an N-terminus comprising the mammalian li-key peptide LRMKLPKPPKPVSKMR (SEQ ID NO: 1) and modifications thereof which retain antigen presentation enhancing activity, a C-terminus comprising an antigenic epitope in the form of a polypeptide or peptidomimetic structure which binds to the antigenic peptide binding site of an MHC class II molecule, and an intervening chemical structure covalently linking the N-terminal and C- terminal components, in a particular embodiment, the hybrid peptides of the present invention comprise influenza MHC class II epitopes identified herein as being effective in generating an immune response and provide immunity to the individual.

La présente invention concerne un polypeptide hybride augmentant la présentation des antigènes par le CMH de classe II. L'hybride a une extrémité N-terminale comprenant le peptide li-key des mammifères LRMKLPKPPKPVSKMR (SEQ ID n° 1 ) et des modifications de celui-ci qui conservent l'activité d'augmentation de la présentation des antigènes, une extrémité terminale C comprenant un épitope antigénique sous la forme d'un polypeptide ou d'une structure peptidomimétique qui se lie au site de liaison du peptide antigénique d'une molécule de classe II du CMH, et une structure chimique intercalaire qui lie de manière covalente les extrémités N-terminale et C-terminale. Dans un mode de réalisation particulier, les peptides hybrides de la présente invention comprennent des épitopes CMH de classe II de la grippe identifiés ici comme étant efficaces dans la production d'une réponse immunitaire et confèrent une immunité à l'individu.

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