Lid element

C - Chemistry – Metallurgy – 12 – M

Patent

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Details

C12M 1/34 (2006.01) C12M 3/00 (2006.01)

Patent

CA 2474866

The invention relates to a lid element that is placed on cell culture vessels in which cells are stored in a liquid medium. The aim of the invention is to provide a lid element which allows to determine the metabolic activities of cells contained in the cell culture vessels by optical measuring methods, and which is easy to handle. According to the invention, the lid element which is placed on the cell culture vessels is provided with light-carrying elements that project into the interior of cavities of the cell vessel when the lid element is placed on the vessel. At least one optically sensitive layer is configured on a front face and/or on the outer periphery of the light-carrying elements that are preferably configured as rod-shaped optical waveguides and allows detection of changing chemical substance concentrations in the cavities.

L'invention concerne un élément couvercle qui est destiné à un récipient de culture de cellules, les cellules étant contenues dans un agent liquide. L'invention vise à proposer un élément couvercle qui permette de déterminer les activités métaboliques des cellules contenues dans le récipient de culture de cellules par des procédés de mesure optique et qui soit facile à manipuler pour le personnel de laboratoire. L'élément couvercle que l'on pose sur un récipient de culture de cellules, présente des éléments conducteurs de rayons lumineux qui pénètrent dans les compartiments du récipient de cellules lorsque l'élément couvercle est posé. Au moins une couche de sensibilité optique est appliquée sur la face frontale et/ou la surface latérale externe des éléments conducteurs de rayons lumineux qui sont, de préférence, réalisés comme guides d'ondes optiques en forme de barres, cette couche de sensibilité optique étant destinée à détecter les changements de concentration d'une matière chimique dans les compartiments.

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