Lifetime-based sensing of sodium and potassium

Details Lifetime-based sensing of sodium and potassium Lifetime-based sensing of sodium and potassium

G - Physics

01

N

G01N 33/20 (2006.01) G01N 21/64 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2325140

A system and method of optically measuring Na+ and K+ in a sample such as blood which contains high concentrations Na+ (up to 160 mM) and K+ (up to 6.5 mM) using a photoluminescent probe having intrinsic analyte-induced lifetime changes. Specifically, the use of lifetime-based sensing of Na+ and K+ at the extracellular concentrations present in whole blood or blood serum. The preferred embodiment uses phase-modulation fluorometry.

L'invention porte sur un système et un procédé de mesure optique du Na?+¿ et de K?+¿ d'un échantillon, par exemple de sang, en contenant de fortes concentrations jusqu'à 160 mM de Na?+¿ et jusqu'à 6,5 mM de K?+¿, en recourant à une sonde photoluminescente présentant des variations intrinsèques de durée de vie sous l'effet de l'analyte. L'invention porte spécifiquement sur l'utilisation de la détection, basée sur la durée de vie, du Na?+¿ et du K?+¿ présents à des concentrations extracellulaire dans du sang entier ou dans du sérum sanguin. L'exécution préférée recourt à la fluorométrie à modulation de phase.

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