C - Chemistry – Metallurgy – 07 – F
Patent
C - Chemistry, Metallurgy
07
F
150/11, 260/210,
C07F 9/40 (2006.01) C07J 41/00 (2006.01) C12Q 1/46 (2006.01) G01N 33/532 (2006.01) G01N 33/542 (2006.01)
Patent
CA 1137077
A B S T R A C T The present invention encompasses a method for determining ligands in test samples comprising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount of ligand in the test sample, said binding protein inactivating the irreversible enzyme inhibitor when bound to the ligand analog portion of the conjugate; inter- mixing an enzyme which is irreversibly inhibited by the ligand analog-irreversible enzyme inhibitor conjugate unbound by the binding protein; and intermixing substrate to the enzyme and monitoring the enzyme substrate reaction. The invention also includes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in practicing the method. Methods and reagents of the present invention are particularly useful in determining drugs, hormones, and the like in biological fluids.
343676
Herrin Thomas R.
Plattner Jacob
Voss Houston F.
Abbott Laboratories
Macrae & Co.
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