Ligand receptor assay employing squarate dyes

Details Ligand receptor assay employing squarate dyes Ligand receptor assay employing squarate dyes

C - Chemistry, Metallurgy

09

B

167/43, 260/8

C09B 23/00 (2006.01) C09B 23/01 (2006.01) C09B 53/00 (2006.01) C09B 57/00 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01)

Patent

CA 1304076

ABSTRACT OF THE DISCLOSURE Novel assays for ligands and receptors employing novel compounds that are conjugates of squarates dyes and members of a specific binding pair (sbp) arc disclosed. The sbp members are selected from the group consisting of ligand and its complementary receptor. The sbp member is covalently or non- covalently bound to the squarate dye, which usually has an absorption maximum greater than 600 nanometers. The novel conjugates are employed in assays for determining the presence or amount of an sbp member analyte in a sample suspected of containing such analyte. Kits comprising such novel conjugates are also disclosed.

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