Ligase/polymerase-mediated primer extension of single...

Details Ligase/polymerase-mediated primer extension of single... Ligase/polymerase-mediated primer extension of single...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2182517

A method is provided for determining the identity of a nucleotide at a preselected site in a nucleic acid molecule. The method involves the incorporation of a nucleoside triphosphate that is complementary to the nucleotide present at the preselected site onto the terminus of a primer molecule, and their subsequent ligation to a second oligonucleotide. The reaction is monitored by detecting a specific label attached to the reaction's solid phase or by detection in solution.

Procédé permettant de déterminer l'identité d'un nucléotide situé sur un site présélectionné dans une molécule d'acide nucléique, consistant à incorporer un triphosphate de nucléoside complémentaire au nucléotide présent sur le site présélectionné, sur la terminaison d'une molécule d'amorce, et à lier lesdits éléments à un second oligonucléotide. La réaction est contrôlée par la détection d'une étiquette spécifique attachée à la phase solide de la réaction ou par la détection en solution.

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