Ligation amplification

Details Ligation amplification Ligation amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2674766

Provided herein are methods and agents for ligation-based exponential ribonucleic acid amplification followed by detection using a nucleic acid polymerization reaction employing terminal-phosphate-labeled nucleotides including three or more phosphates as substrates for nucleic acid polymerase.

La présente invention concerne des procédés et des agents pour une amplification exponentielle d'acide ribonucléique basée sur la ligature suivie d'une détection utilisant une réaction de polymérisation d'acide nucléique utilisant des nucléotides marqués par un phosphate terminal comprenant trois ou plus de phosphates comme substrats pour la polymérase d'acide nucléique.

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