Ligation-based rna amplification

Details Ligation-based rna amplification Ligation-based rna amplification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2592425

Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.

L'invention concerne des procédés d'amplification, de purification et de détection de séquences d'acides nucléiques, notamment l'ARN. Un mode de réalisation concerne l'hybridation et la ligature subséquente d'une structure d'acides nucléiques à une séquence d'acides nucléiques à manipuler. Pour mettre en oeuvre les procédés, il faut que la structure d'acides nucléiques comprenne une région à double brin et une région à brin unique. La région à brin unique est complémentaire de la séquence d'ARN étudiée. La région à double brin peut également comprendre des fonctionnalités supplémentaires utilisées ensuite dans le procédé.

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