Light modulated microarray reader and methods relating thereto

Details Light modulated microarray reader and methods relating thereto Light modulated microarray reader and methods relating thereto

G - Physics

01

N

G01N 21/00 (2006.01) G01N 21/25 (2006.01) G01N 21/64 (2006.01) G01N 35/00 (2006.01) G02B 26/02 (2006.01) G02B 27/02 (2006.01) G06F 17/10 (2006.01)

Patent

CA 2452693

Microarray readers and methods that compensate for target spots that are too dim or too bright for the microarray reader to accurately measure. The readers adjust the amount of light directed at or received from specific non- acceptable target spots, such that dim spots receive more excitation light and overly bright spots receive less. This increases or decreases, respectively, their measured brightness, which in turn effectively increases the range over which a microarray reader can accurately measure the spots, and can improve the signal-to-noise ratio and other aspects of the measurements.

L'invention a trait à des lecteurs de microréseaux et à des procédés qui compensent les points cibles dont le manque ou l'excès de luminosité empêche le lecteur de microréseaux de les mesurer de manière exacte. Les lecteurs ajustent la quantité de lumière dirigée sur des points cibles non-acceptables spécifiques ou reçue de ces derniers, de sorte que les points sombres reçoivent davantage de lumière d'excitation et les points excessivement clairs en reçoivent moins. Ce dispositif augmente ou réduit respectivement leur luminosité mesurée, ce qui a pour effet d'augmenter efficacement le spectre sur lequel un lecteur de microréseaux peut mesurer les points de manière précise, et peut améliorer le rapport signal sur bruit ainsi que d'autres aspects relatifs aux mesures.

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