Light scatter-based immunoassay without particle self...

G - Physics – 01 – N

Patent

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Details

IPC codes

G01N 33/546 (2006.01) G01N 15/14 (2006.01) G01N 21/47 (2006.01) G01N 21/49 (2006.01) G01N 21/53 (2006.01) G01N 33/543 (2006.01) G01N 33/553 (2006.01) G01N 15/02 (2006.01)

Type

Patent

Patent number

CA 2151060

Description

A homogeneous immunoassay method for the simultaneous determination of one or more antibody, antigen or hapten analytes in a fluid sample, that comprises the quantification of the effect of said analytes on the statistical changes in a dimension of a light scatter pulse height distribution histogram of relatively large diameter monodisperse binding molecule-coated polymeric microspheres induced by the binding to said microspheres of polydisperse binding molecule-coated colloid metal particles of relatively small diameter. For simultaneous assays of multiple analytes, different diameter or refractive index microspheres are assigned to each analyte. The assay may be used in forward binding, displacement, inhibition, and competition type systems, with the direction of the change in histogram dimension depending on the system. A convenient dimension to measure is the normalized peak width of a graphical representation of the histogram. For simultaneous assays for multiple analytes, a monodisperse polymeric microsphere of unique diameter or refractive index is dedicated to each analyte, so as to generate multiple histograms, one for each analyte. Polydisperse binding molecule-coated colloid metal particles, when used in effective concentrations, will also serve as a scavenger means to reduce the interfering effects of non-specific substances in the analyte-containing fluid samples, particularly when said samples are of biological origin. An improved sheath flow cell for a FPA is also described.

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