Linear and circular expression elements

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/79 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) C12N 15/82 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2365981

The present invention relates to linear expression elements (LEEs) and circular expression elements (CEEs), which are useful in a variety of molecular biology protocols. Specifically, the invention relates to the use of LEEs and CEEs to screen for gene function, biological effects of gene function, antigens, and promoter function. The invention also provides methods of producing proteins, antibodies, antigens, and vaccines. Also, the invention relates to methods of making LEEs and CEEs, and LEEs and CEEs produced by such methods.

La présente invention concerne des éléments d'expression linéaires (EEL) et des éléments d'expression circulaires (EEC) qui sont utiles dans divers protocoles de biologie moléculaire. De manière plus spécifique, cette invention porte sur l'utilisation de EEL et de EEC pour dépister la fonction génique, les effets biologiques de la fonction génique, des antigènes et le fonctionnement des promoteurs; ainsi que des procédés de production de protéines, d'anticorps, d'antigènes et de vaccins; et que des procédés de préparation de EEL et de EEC, et les EEL et les EEC produits au moyen de ces mêmes procédés.

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