Linear donor constructs for targeted integration

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2720903

Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.

L'invention concerne des molécules donneuses linéaires comprenant des bras d'homologie de 50-750 paires de bases (par exemple, 50-100 paires de bases) flanquant une ou plusieurs séquences d'intérêt. Les molécules donneuses et/ou des compositions comprenant ces molécules peuvent être utilisées dans des procédés d'intégration ciblée d'une séquence exogène dans une région d'intérêt spécifiée dans le génome d'une cellule.

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