C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/18 (2006.01) A61K 39/395 (2006.01) A61P 35/00 (2006.01) C12N 15/13 (2006.01) C12N 15/63 (2006.01) C12N 15/70 (2006.01)
Patent
CA 2561826
This invention refers to a linear single chain recombinant trispecific antibody (scTsAb). This trispecific antibody is composed of an anti-carcinoembryonic antigen (CEA), an anti-CD3, and an anti-CD28 (CEA-scTsAb). More specifically, a Fc linking peptide fuses the anti- CEA single chain fragment (scFv) to the anti-CD3 scFv, and an HSA linking peptide fuses the anti CD3 scFv to the anti-CD28 heavy variable chain (V H) in tandem. A c-myc-tag and/or (His) 6-tag is attached to the C terminal of the CEA-scTsAb either alternatively or simultaneously. This invention also refers DNA sequence coding for the said scTsAbs, plasmids containing the said sequences, and host cells containing the said plasmids. These invented scTsAbs are intended for use in the development of therapeutic drug candidate for treatment of human colon cancer.
L'invention concerne un anticorps trispécifique monocaténaire recombiné, fait à partir d'un anticorps antitumoral, d'un fragment Fc d'un anticorps humain anti-CD3, d'un fragment HSA et d'un anticorps humain anti-CD28. L'invention concerne en particulier CEA-TsAb, un anticorps trispécifique monocaténaire recombiné anti-CEA, anti-CD3 et anti-CD28, formé de trois fragments d'anticorps en tandem (scFv anti-CEA, scFv anti-CD3 et anticorps anti-CD28 à domaine unique) liés par deux fragments (fragments Fc et HSA), et auquel une étiquette c-myc ou histidine ((His)6) peut être ajoutée à l'extrémité C. L'invention concerne aussi un procédé de construction, d'expression et de purification de l'anticorps ; et la séquence d'ADN codée de l'anticorps, des vecteurs d'expression et des cellules hôtes pour les vecteurs.
Huang Hualiang
Lin Qing
Piao Jinhua
Wang Xiangbin
Zhao Baofeng
Beijing Abt Genetic Engineering Technology Co. Ltd.
Cassan Maclean
Dongguan Haofa Biotechnology Developmental Co. Ltd.
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