Linear vectors, host cells and cloning methods

Details Linear vectors, host cells and cloning methods Linear vectors, host cells and cloning methods

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C12N 9/90 (2006.01)

Patent

CA 2636816

Linear vectors derived from bacteriophage of E. coli and host cells suitable for cloning are provided. The linear vectors include a left arm comprising a left telomere and a first selectable marker, a right arm comprising a right telomere and a second selectable marker and a cloning region located between the left arm and the right arm. Optional further components of the vector include transcriptional termination sequences, multiple cloning sites and reporter stuffer regions.

La présente invention concerne des vecteurs linéaires dérivés d'un bactériophage de E.coli, ainsi que des cellules hôtes adaptées au clonage. Ces vecteurs linéaires comprennent un bras gauche présentant un télomère gauche et un premier marqueur de sélection, un bras droit présentant un télomère droit et un second marqueur de sélection, ainsi qu'une région de clonage qui est située entre le bras gauche et le bras droit. Des séquences de terminaison transcriptionnelle, des sites de clonage multiple et des régions de remplissage de gène rapporteur constituent d'autres composants éventuels du vecteur.

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