Linoleate and linolenate-lipoxygenase mutants

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2367258

The invention relates to a method for producing plant lipoxygenases with modified position specificity, to the lipoxygenases obtained using this method and to the use of said lipoxygenases for hydroperoxylating substrates. In particular, the inventive LOXs provide the first means of producing novel .gamma.-linolenic acid derivatives on a large scale. To this end, .gamma.- linolenic acid is incubated with the inventive LOXs in suitable conditions as a substrate. The .gamma.-linolenic acid is then hydroperoxylated according to the LOXs mutants used, preferably in position 6 or position 9 or positions 6 and 9.

La présente invention concerne un procédé permettant de produire des lipoxygénases végétales présentant une spécificité de positions modifiée, ainsi que la lipoxygénase obtenue selon ce procédé et l'utilisation de celle-ci pour l'hydroperoxylation de substrats. Les LOX (lipoxygénases) selon l'invention permettent en particulier, pour la première fois, la production de nouveaux dérivés d'acide .gamma.-linoléique en grandes quantités. A cet effet, de l'acide .gamma.-linoléique est incubé, en tant que substrat, avec les LOX selon l'invention, dans des conditions appropriées. Selon le mutant de LOX utilisé, il se produit alors une hydroperoxylation de l'acide .gamma.-linoléique, de préférence à la position 6 ou à la position 9, ou aux positions 6 et 9.

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