Lipase determination method and reagent

Details Lipase determination method and reagent Lipase determination method and reagent

C - Chemistry, Metallurgy

12

Q

150/3.2, 150/15.

C12Q 1/44 (2006.01)

Patent

CA 1128843

ABSTRACT OF THE DISCLOSURE An improved reagent and method for determination of lipase in biological fluids utilizes an S-acyl compound as the substrate and a chromogenic sulfhydryl reagent to develop a measureable color. The improved reagent and method include a solid, water soluble pseudocholinesterase inhibitor, preferably eserine salicylate. Albumin may also included to increase sensitivity. Lipase is measured by incubating a specimen with the S-acyl compound, chromogenic sulf- hydryl reagent and pseudocholinesterase inhibitor; terminating the incubation by adding a non-ionic sur- factant; measuring the resulting color directly, and comparing the color to that obtained with a chemical standard solution containing an S-(2-aminoalkyl) iso- thiouronium salt.

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