Lipid-modified serum free media

Details Lipid-modified serum free media Lipid-modified serum free media

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01) C12N 5/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2170761

Methods are provided for producing serum-free. animal cell culture media which have enhanced cell culture properties. such as cell growth, cell viability and protein production properties. The cell culture properties of standard serum-free media formulations, which include compositions such as inorganic salts, glucose, fatty acids, amino acids and vitamins, can be enhanced according to the methods of the invention, by providing the media with an additive. The additive in one embodiment can be produced by the acid or base catalyzed hydrolysis of a phosphoglyceride.

Méthodes pour produire des milieux de culture de cellules animales sans sérum ayant des caractéristiques améliorées, notamment en ce qui concerne la croissance cellulaire, la viabilité des cellules et la production de protéines. Il est possible d'améliorer les milieux sans sérum standard pour la culture cellulaire, notamment ceux qui utilisent des sels inorganiques, du glucose, des acides gras, des acides aminés et des vitamines, au moyen des méthodes présentées dans le brevet en ajoutant un additif aux milieux. L'additif dans un modèle peut être produit par l'hydrolyse d'un phosphoglycéride catalysé par un acide ou une base.

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