Lipid-vesicle-surface assay reagent and method

G - Physics – 01 – N

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G01N 33/544 (2006.01) G01N 33/554 (2006.01)

Patent

CA 1239582

LIPID-VESICLE-SURFACE ASSAY REAGENT AND METHOD ABSTRACT An improved enzyme immunoassay reagent composed of lipid vesicles coated with a mobile surface array of ligand and enzyme molecules is described. The reagent is adapted for use in an enzyme immunoassay in which the reagent partitions between a separable support and a liquid phase, in proportion to the amount of analyte present. The amount of reagent in the liquid or support phase is determined by measuring the enzyme activity associated with the reagent in that phase. Also described is an immunoassay employing such a reagent.

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