C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/20 (2006.01) C12N 15/55 (2006.01) C12Q 1/34 (2006.01) C12Q 1/44 (2006.01)
Patent
CA 2569659
The present invention relates to a method for measuring the activity of a lipolytic enzyme comprising measuring the pH of the gas phase of the enzymatic reaction between the lipolytic enzyme and a substrate, wherein the substrate comprises an ester bond between a C1-C10 carboxylic acid and an alcohol. Furthermore, it relates to a method for screening a library of polypeptide for a lipolytic enzyme of interest comprising testing the library in said method.
Cette invention concerne un procédé permettant de mesurer l'activité d'une enzyme lipolytique, lequel procédé consiste à mesurer le pH de la phase gazeuse de la réaction enzymatique entre l'enzyme lipolytique et un substrat, lequel substrat comprend une liaison ester entre un acide carboxylique C¿1?-C¿10? et un alcool. en outre, cette invention concerne également un procédé permettant de cribler une banque de polypeptides pour une enzyme lipolytique présentant un intérêt, lequel procédé consiste à tester la banque susmentionnée.
Abel Gernot
Bjornvad Mads Eskelund
Dimock Stratton Llp
Novozymes A/s
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